Elizabeth Murray
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Dr. Murray's ten tips for scientific presentations

10/27/2019

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Want to improve your presentation skills? Are you excited to learn better methods to communicate research? We scientists do cool stuff, and we want (and need) to convey our work to others. I have some hints to share. 
This past year, I've read lots of books, searched articles, and watched tutorials -- all in the quest of improving my scientific communication skills. Here are some ideas and tips I've been incorporating into my presentations. Maybe you want to try something new, too?!
#1. Change your mindset.
Begin with a 'conversation' mentality. You're giving a talk, and not "giving your slides". You will be actively communicating with the audience, using your narrative & an engaging method of delivery so that they will benefit from your talk. Think of slides as a tool to support your presentation.

Concrete step: For many of us, the default framework is to set up each slide in a typical "title / bullet point text / supporting visual". As you develop a presentation, try adopting a mentality where you use slides to support your conversation with the audience. Imagine constructing the slide outwards from the center of the page (build out from the message), instead of assembling a slide top-down (titles and lists).
#2. Develop your message & bring the audience along.
What do you want people to remember when they leave the room? You have something valuable to give to the audience, and it will take some effort to figure out how to best deliver it to them. What info or data best contributes to the takeaway message? You'll need to make tough editing decisions to remove or condense parts that don't pull their weight.

You're responsible for guiding the audience through the presentation. Make it easy for them. For instance: refresh people's memory of what you explained 5 slides ago, repeat important information in a new way, and connect your ideas logically. I got this advice almost 15 years ago, but I don't think it stuck back then. Now it's sticking.


​bonus exercise: What aspects of your professional identity & research will you emphasize for your talk?
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Try this exercise to clarify your delivery style:  "After my presentation, [audience] will realize [objective], so they will [desired outcome] and think of me as [desired impression].

Example 1: "After my presentation, academic faculty in the audience will realize I am an independent scientist & a creative problem-solver, so they will consider me as a candidate to fill their postdoc position and will envision me as a beneficial addition to their lab."

Example 2: "After seeing my presentation, my colleagues will realize I am a talented bioinformatician who is knowledgeable about the latest methods, so they will hold me in high esteem and think of me as an expert in this methodological field. They will seek me out as a collaborator."
adapted from: ROAM [Reader, Objective, Aim, iMpression] (Bernhoff, 2016)
"guide your audience" advice from Dr. Yoonseong Park (Kansas State, Entomology)
#3. Find your narrative.
Creating narratives is natural for humans. We've evolved to respond to stories. Amazingly, during storytelling, the brains of the storyteller and the listener are activated in the same patterns (Janzer, 2018). So, consider to make use of this tool of evolution to help you deliver information in a way the audience is equipped to receive!

Check out my previous blog post on the use of the narrative strategy in science, based on work by Randy Olson.
#4. Tell your audience what they'll know by the end of your talk.
Give a promise for a new piece of knowledge that the audience can grab onto and walk out of the room with. Put this somewhere early on, where it feels right. Maybe at the very start, or after the introduction. What will you give to your audience? Then they'll know what to pay attention for! This is a great way to make sure you can connect the importance of your presentation to the audience's needs. 
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Related: Don't squander the first minute of your talk. Get your audience committed to you & your topic. What can you say in the first 10 seconds to engage them?
(ideas from Donovan, 2013 and Anderson, 2016)
#5. Group by threes. 
The magic number is 3. A list of 2 is not a list. And a list of 4 gets too long for people to hold in their mind. I'm not saying you need three things on a slide. But... what about thinking about 3 objectives, 3 key points, or, for longer talks, 3 related projects you'll share with the audience? (idea from Donovan, 2013)
Success story: I shared my "3-pronged approach" strategy with a friend, who used it in a presentation involving the restaurant business. She had a photo of a 3-pronged fork next to her list describing her 3-pronged approach. They loved it -- rave reviews. True, we can't usually seamlessly incorporate fork photos into our presentations, but keep your 3-pronged strategy handy!
#6. Cut down the text on your slides. 
So hard! I'm still working on this one. But no one in the audience wants to read a bunch of stuff, or listen to you read a bunch of stuff. Can you break up a list into multiple slides? Or use icons/illustrations to represent those points?

Dr. Echo Rivera recommends using visuals instead of text. A good gauge of text: don't use >50 words on a slide.
#7. Mix it up & give a visual break: speak while displaying a blank screen.
I really like this one. You choose to give the audience a rest from looking back and forth from you and the screen or focusing their attention only on the slides & not you as the speaker. Use this as a chance to hammer home a message or switch gears and introduce a new topic.
​

Embrace the 'eyes on me' mentality! 

Adapted from advice to use black slides in the great tips from Dr. David L Stern. (Check them all out!) I tried using the black screen approach in a practice talk and it made the audience uncomfortable. They thought something went wrong & wondered when I'd notice. Now, for my 'white screen' approach, I keep the slide blank while I talk (or cheat a bit and put up the new topic keyword on the otherwise-blank slide).
[White is my background color for slides unless I'm using a full-page image.]
#8. Connect to audience by small changes in your phrasing. 
Consider posing a rhetorical question to the audience or asking them to predict a result (you don't need them to answer out loud, but someone may!). Be sure to use "you" singular when addressing the audience. Don't let them disappear into an anonymous "you all" and not take responsibility for upholding their side of the interaction. Treat your presentation as a conversation. (suggested by Donovan, 2013)
​

Second, use a mindful "I" phrase to emphasize a place in your presentation where you want the audience to pay attention. People will perk up when you connect with them this way. Maybe state: "I wasn't expecting this result and therefore I decided to ...", or, "I realized this is the most important reason that we should ...". 
#9. Meaning before details.
It's usually good to state your message before you describe the nitty-gritty. The audience should realize WHY you're leading them a certain direction, so that they aren't struggling to carry all the details in their head without knowing the destination. If you don't first tell the audience the intention of a litany of information, they focus too much attention on anticipating the conclusion so they can put it all together. 
​
This won't apply to every slide/subject, and sometimes you want a slow build-up & exciting reveal. But typically: lead with the message.


advice from Dr. Rayna Bell, California Academy of Sciences (at a practice talk of mine)
"Meaning before details" from J. Medina (Janzer, 2018)
#10. Be smart with how you use your slides.
Slide design is a big topic, and I won't cover it here. But of course, you'll want to think about your slide use & design and how that will help you spread your science message! A few thoughts:

do get comfortable with negative space on slides: Intentional empty space is fine! But at the same time, always make sure your graphics are large and clear -- visuals are important, and you put them there for a reason, so make them work for you!

don't use a laser pointer: Design your slides so that you don't NEED to use a pointer. Instead, build on your slides using animation, so that arrows or emphases pop up when you need it.

do use presenter mode, especially if you feel nervous about giving talks: Maybe this is an obvious one? I only started using presenter mode last year, and I have definitely benefited. There's a timer so you can use it to stay on schedule. But more importantly, I'd recommend you make good use of the notes section, since you can see your typed notes while you give a talk. Write and practice (and ruthlessly edit!) a script. Or write up and refer to your notes for topic prompts and transitions to help keep you on track and give a smooth and polished delivery.
Keyboard shortcut to get into presenter mode at the current slide: (SHIFT+ALT+F5 /or SHIFT+ALT+FN+F5).
Bonus for job talks: #11. Brand yourself at the beginning.
In a job talk, you are introducing yourself as well as presenting your research. The audience is interested to see you in a broader view than just the research you include, and you can help them by giving this context. Can you summarize your professional identify in one sentence?

Right: Slide #2 from my recent job talk. This way, people got a sense of what I was trying to do in my research life. An oral statement would've worked, too.
I envisioned my 'mission statement'. Yes it was broad, but it is supported in the research and story line following in my presentation. I wanted to make it easy for the audience to see who I am, so that my work would be interpreted in the context of my broader professional identity.
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Telling people what I do so that they know from the get-go.
I'd love to hear your suggestions or feedback on any of these hints!
references:
Anderson, C. (2017). TED Talks: The Official TED Guide to Public Speaking.​ Mariner Books, 288 p.
Bernoff, J. (2016) Writing Without Bullshit: Boost Your Career by Saying What You Mean. HarperBusiness, 304 p.
Donovan, J. (2013) How to Deliver a TED Talk: Secrets of the World's Most Inspiring Presentations. McGraw-Hill Education, 240 p.
Janzer, A. (2018) Writing to be Understood: What Works and Why. Cuesta Park Consulting, 219 p.

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Grab & Graph GBIF Biodiversity Data Using R

6/2/2019

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R + GBIF = rgbif
Here is a tutorial for the R package 'rgbif'. This allows you to access specimen information in the Global Biodiversity Information Facility database. GBIF has hundreds of millions of species occurrence records from around the globe, open for anyone to use.
How did these records get into GBIF in the first place? The data come from many sources -- various museums, universities, and other institutions. Specimen label data has been recorded and digitized in spreadsheets, and this info is contributed to GBIF. I wrote a script in R to demonstrate how to import records for a desired taxon found in a region, and then I use R functions to display the data. 
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Orasema
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Lophyrocera
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Kapala

As shown in the tutorial, I accessed GBIF records for all the Eucharitidae collected in the US. Accessing the data is rather straightforward. Manipulating the data for display takes a few more functions. The records at right are grouped by genus, with any N/As renamed as 'no genus ID'. The genera were then sorted by their number of records. I didn't clean the data, which would be smart to do if you're using it for a project.
rgbif R script, opens in a new window
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Below I display the number of eucharitid records per year in two different ways.
On the left is a violin plot, which I think is a neat way to look at how data are distributed. The width of the violin is relative to the number of yearly records -- not the actual values, but a probability density distribution. The boxplot within shows the median value as a white dot. I excluded zeros here, so we aren't seeing the years where no Eucharitidae were recorded (this keeps the script slightly simpler).
On the right is a chronological view of records per year, excluding years with zero records. I've distinguished the years having more than ten records by using a darker bar color.
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I enjoyed exploring rgbif and thought it turned into a good introduction to how to plot data from a biodiversity database. I hope you try it out, too! Also, here's another rgbif tutorial I recently found that looks pretty useful; it focuses more on the data manipulation than the data display.
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Science Stories

3/3/2019

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And. But. Therefore. 
These three words give a clear format for telling a narrative, writing a scientific abstract, or organizing a conference talk. After reading "Houston, We Have a Narrative", I have been incorporating this structure into my scientific work. Have you tried out 'ABT'?
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... I realized that the paper towel promise of a life-changing book was not even an exaggeration.
About a year ago, I walked into the lab and found this scene (shown at left) at the meeting table. 
​
From this paper-towel prompting, I read "Houston, We Have a Narrative" over the next two nights and can now say that it has made an impact on the way I set up presentations and how I think about my writing.
Some scientists may shy away from visualizing their research as a story to be told, but Olson argues that a narrative is what humans are built to respond to. He defines a narrative (p 182) as "a series of events that happen along the way in the search for a solution to a problem". A narrative is not a fictional piece of work, and it doesn't have to be non-scientific. I'd recommend checking out the book!

If you attended the Entomological Society of America meeting in Vancouver (Nov. 2018), maybe you saw Randy Olson's plenary talk. He explained a newer concept he developed called the 'Narrative Index' where he calculates the ratio of BUTS to ANDS (x100) in speeches or writings. Higher is better & shows a stronger narrative. For instance, he calculated the Lincoln-Douglas debates (Lincoln was better with a mean score of 20, instead of Douglas's score of 9.29). At the bottom of his web page, you can see what Olson has calculated for other presidents, and what he finds as Donald Trump's scores.
reference: Olson, R. (2015) Houston, we have a narrative: Why science needs story. University of Chicago Press.
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Pollen feeding and diversification of bees

11/24/2018

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Did you know that bees evolved from within a group of solitary, carnivorous wasps, approximately 120 million years ago? Today, pollinivory – the consumption of pollen – is a defining feature of bees (aptly named Anthophila; the flower lovers). 
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Though it's been a common assumption that the bees diversified due to their switch to pollen feeding, in a recent study, we show it isn't the full story. Co-authors are Silas Bossert & Bryan Danforth.

​We have a short and sweet paper freely available in the November 2018 issue of Biology Letters. ​

I was excited that a bee photo taken by my little brother was chosen as the cover image of the issue. This Megachile bee is from Minnesota, USA.
In this study, one of the core concepts is the role of the ‘key innovations’ underlying biodiversity patterns. A key innovation is an evolutionary novelty which is believed to contribute to the success of a group. (For example, across all insects, some of the major transitions that are hypothesized to be key innovations include the origin of wings/flight, and the origin of complete metamorphosis.)
 
Under the traditional definition of innovation, we would predict that a lineage with the key trait is more speciose than related groups lacking this key trait. The switch to pollen feeding has been assumed to be a key innovation of bees: the bees arose from within a group of carnivorous wasps, were able to exploit a new food resource, and today are extremely species rich.
 
Our finding that not all bees exhibit a high diversification rate challenges conventional thought that the switch to pollinivory is directly responsible for increased bee diversity. We found that some of the earliest-originating bees did not partake in the diversification upswing. These results indicate that pollen feeding was an important evolutionary switch, but does not fully explain the diversity we see today. We postulate that other complementary innovations, such as a generalist host-plant diet, influenced the tremendous diversification of the major bee lineages.
 
On a broader scale, this study contributes to an area of interest in the insect scientific community, of investigating whether diet shifts to plant-feeding contribute to higher diversity. Pollinivory is a specialized form of herbivory. Classic and contemporary studies have found a general pattern across insects that herbivory increases diversification (i.e., Mitter et al. 1988 & Wiens et al. 2015). We found that the evolutionary shift to plant-feeding contributed to bee diversification, but our results indicate it is not directly responsible for the increase in diversification rates. 
The Cornell Chronicle published an article on our paper!
Read it here: Study Challenges Widely Held Assumption of Bee Evolution.
citation: Murray, E.A. Bossert, S., Danforth, B.N. (2018)  Pollinivory and the diversification dynamics of bees. Biology Letters, 14, 20180530. DOI: 10.1098/rsbl.2018.0530
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Combining UCEs and transcriptomes

10/30/2018

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Phylogenomic methods are incredibly popular, and there are various baits and probes for capturing different pieces of the insect genome for analysis. One of the next aspects of 'big data' will be to develop ways to combine all of these sources!
My previous post covered a method for visualizing data on tree tips -- specifically I showed a plot of the number of loci recovered after doing a quick combination of genomes, transcriptomes, and UCEs (using the Phyluce pipeline). I'm happy to write that our research paper on the combination of phylogenomic data has been published in Molecular Phylogenetics and Evolution. Bossert et al. 2019 report successful results when three types of datasets are combined for Apidae (the largest bee family).
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Bossert et al. figure 1. Graphical summary of the workflow developed for combining genome, transcriptome, and UCE data, exemplified for the widely shared HIPK2 gene of the honey bee.

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Summary figure of the phylogeny of the bee family Apidae. The topology was in agreement with many of the previously published higher-level groupings. Despite widespread acknowledgement of a non-monophyletic Apinae, no taxonomic changes were proposed until now. Find the revised Apidae classification in Appendix A!
The trick to getting the transcriptomes to align without an excessive amount of error was to use the available genomes as a backbone. A transcriptome of the same length as a UCE in reality covers a longer region of the genome due to the fact that introns are excluded in these coding regions. By grabbing a long piece of the genome, exons at the ends of the transcriptome fragments could 'stretch out' and would not be misaligned with the ends of the UCEs.

Around the time our work came out, another group published a paper with a similar objective, using the Hemiptera UCE probe set. These authors had an alternative approach to combining transcriptomes and UCEs -- using  tblastx to search for homologous loci of UCEs in transcriptomes.
references:
Bossert, S., Murray, E.A., Almeida, E.A.B., Brady, S.G., Blaimer, B.B. & Danforth, B.N. (2019) Combining transcriptomes and ultraconserved elements to illuminate the phylogeny of Apidae. Molecular Phylogenetics and Evolution, 130, 121-131. doi.org/10.1016/j.ympev.2018.10.012 
Kieran, T.J., Gordon, E.R., Forthman, M., Hoey-Chamberlain, R., Kimball, R.T., Faircloth, B.C., Weirauch, C. and Glenn, T.C. (2019) Insight from an ultraconserved element bait set designed for Hemipteran phylogenetics integrated with genomic resources. Molecular Phylogenetics and Evolution, 130, 297-303. 
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Elizabeth A. Murray, ​PHYLOGENETICS AND EVOLUTION of Hymenoptera

@PhyloSolving  |  e.murray @ wsu.edu
  • home
  • research
    • phylogenomics in Aculeata
    • bee viruses
    • eucharitid ant parasitoids
  • publications
  • teaching
  • blog